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puc19 vector plasmid  (New England Biolabs)


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    Structured Review

    New England Biolabs puc19 vector plasmid
    Puc19 Vector Plasmid, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1407 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+puc19/pUC19+Vector/10__3390_slash_v18040470-76-41-55
    Average 97 stars, based on 1407 article reviews
    puc19 vector plasmid - by Bioz Stars, 2026-10
    97/100 stars

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    Plasmid Preparation:

    Article Title: Cost-effective PMMA photocatalytic microreactor for nanocatalyst screening and environmental research
    Article Snippet: .. The antibacterial efficacy of the photocatalytic microreactor was assessed using E. coli NEB Stable with plasmid pUC19 (non-pathogenic strain, Catalog No C3040I, New England Biolabs, USA) as a model microorganism. ..

    Article Title: A high-throughput single-molecule platform to study DNA supercoiling effect on protein–DNA interactions
    Article Snippet: .. Vector DNA was prepared by digestion with EcoRI (NEB, R3101S, 2 units/μg DNA) and HindIII (NEB, R3104S, 2 units/μg DNA) of plasmid pUC19 (NEB, N3041S) supplemented with rCutSmart Buffer (NEB, B6004S) for 15 min at 37°C. .. The DNA was purified using PCR (polymerase chain reaction) spin column (Qiagen, 28104).

    Article Title: Establishment of Basic Compatibility Through Pathogen Sulfur Scavenging Is Essential for Laurel Wilt Disease Development
    Article Snippet: .. These two amplicons were introduced into plasmid pUC19 between BamHI and Page 23 of 43 Rollins_MPMI 23 HindIII with the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs) to produce plasmid pUC19_RlEF-Cas9. ..

    Article Title: Reentrant DNA shells tune polyphosphate condensate size.
    Article Snippet: .. Plasmid pUC19 was linearized by using restriction enzymes HindIII (NEB) and XbaI (NEB), purified using the NEB miniprep kit and ligated with a Cy5-labeled oligo duplex. ..

    Article Title: Reentrant DNA shells tune polyphosphate condensate size
    Article Snippet: .. Plasmid pUC19 was linearized by using restriction enzymes HindIII (NEB) and XbaI (NEB), purified using the NEB miniprep kit and ligated with a Cy5-labeled oligo duplex. ..

    Article Title: Human and bacterial genetic variation shape oral microbiomes and health
    Article Snippet: Clones were screened for sequence errors before plasmid preparation using Plasmid Plus Midi Kit (Qiagen, catalogue no. 12943). .. Plasmid pUC19 (New England Biolabs, catalogue no. N3041S) was used as a negative control. .. A total of 15 μg of each plasmid was lipofected into separate 10 cm plates of HEK293T (Takara, catalogue no. 632180) cells at ~70% confluence using 30 μl of Lipofectamine 3000 (Invitrogen, catalogue no. L3000015).

    Article Title: A high-throughput single-molecule platform to study DNA supercoiling effect on protein-DNA interactions.
    Article Snippet: .. V ector DNA was prepared by digestion with EcoRI (NEB, R3101S, 2 units / μg DNA) and HindIII (NEB, R3104S, 2 units / μg DNA) of plasmid pUC19 (NEB, N3041S) supplemented with rCutSmart Buffer (NEB, B6004S) for 15 min at 37 ◦C. .. A BsmI site was introduced into the plasmid by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB, E0554), following the manufacturer’s protocol.

    Article Title: Human and bacterial genetic variation shape oral microbiomes and health.
    Article Snippet: Clones were screened for sequence errors before plasmid preparation using Plasmid Plus Midi Kit (Qiagen, catalogue no. 12943). .. Plasmid pUC19 (New England Biolabs, catalogue no. N3041S) was used as a negative control. .. A total of 15 μg of each plasmid was lipofected into separate 10 cm plates of HEK293T (Takara, catalogue no. 632180) cells at ~70% confluence using 30 μl of Lipofectamine 3000 (Invitrogen, catalogue no. L3000015).

    Cloning:

    Article Title: Establishment of Basic Compatibility Through Pathogen Sulfur Scavenging Is Essential for Laurel Wilt Disease Development
    Article Snippet: .. These two amplicons were introduced into plasmid pUC19 between BamHI and Page 23 of 43 Rollins_MPMI 23 HindIII with the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs) to produce plasmid pUC19_RlEF-Cas9. ..

    Purification:

    Article Title: Reentrant DNA shells tune polyphosphate condensate size.
    Article Snippet: .. Plasmid pUC19 was linearized by using restriction enzymes HindIII (NEB) and XbaI (NEB), purified using the NEB miniprep kit and ligated with a Cy5-labeled oligo duplex. ..

    Article Title: Reentrant DNA shells tune polyphosphate condensate size
    Article Snippet: .. Plasmid pUC19 was linearized by using restriction enzymes HindIII (NEB) and XbaI (NEB), purified using the NEB miniprep kit and ligated with a Cy5-labeled oligo duplex. ..

    Negative Control:

    Article Title: Human and bacterial genetic variation shape oral microbiomes and health
    Article Snippet: Clones were screened for sequence errors before plasmid preparation using Plasmid Plus Midi Kit (Qiagen, catalogue no. 12943). .. Plasmid pUC19 (New England Biolabs, catalogue no. N3041S) was used as a negative control. .. A total of 15 μg of each plasmid was lipofected into separate 10 cm plates of HEK293T (Takara, catalogue no. 632180) cells at ~70% confluence using 30 μl of Lipofectamine 3000 (Invitrogen, catalogue no. L3000015).

    Article Title: Human and bacterial genetic variation shape oral microbiomes and health.
    Article Snippet: Clones were screened for sequence errors before plasmid preparation using Plasmid Plus Midi Kit (Qiagen, catalogue no. 12943). .. Plasmid pUC19 (New England Biolabs, catalogue no. N3041S) was used as a negative control. .. A total of 15 μg of each plasmid was lipofected into separate 10 cm plates of HEK293T (Takara, catalogue no. 632180) cells at ~70% confluence using 30 μl of Lipofectamine 3000 (Invitrogen, catalogue no. L3000015).



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